The advantage of a spore syringe is that this spore water can be used to inoculate the substrate of your choice. It contains sterile water with hydrated spores in it. This method is not the best as concentrated populations of spores are grouped together.Ī spore syringe is a bit trickier to make.In this method, the spore print can be folded and rubbed together so that spores drop onto nutrified agar media.See page 93-95 of Paul Stamets’ Growing Gourmet and Medicinal Mushrooms, 3rd edition for great photos of this process.Colonies of germinating spores can then be subcultured into more petri dishes.5 to 15 days later, your spores (should) germinate according to the streaking pattern!.Streak the tip into an “S” pattern across the surface of the petri dish.Touch the spore print with the scalpel tip to collect hundreds of spores.This way the scalpel gets covered with a moist and adhesive layer of media for better spore attachment. Sterilise a scalpel by passing it through a flame, then cooling it in the “receiving” nutrified agar.Germinating the spores from your spore print is best done on nutrified agar plates in a glove box setup, as with tissue/agar cloning.Īlternatively, a spore syringe can be made, but this is a bit more tricky. Glass allows for easy future use of spore as well as observation without risk of contamination.Seal the three remaining edges with more tape, creating a “Spore Booklet”.Remove, dry and store mushrooms for later reference purposes.Place mushroom caps on top of both plates for 12 to 24h, covered with bowl.Create a bind between both plates by joining the two plates with duct tape.Wash glass with soapy water, dry with wipe, clean with alcohol.Can be stored at room temperature for years, in a dark and cool location.Pick up spore printed paper with clean tweezers and put in a ziplock bag.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |